Problem
In microbiome projects, planning is critical to getting meaningful results. Without experience in microbiome study design, important decisions around sampling and methodology may be made too early or on too little evidence. This can lead to project designs that do not produce clear or conclusive results. A common challenge is that the right sequencing method may be identifiable, while key questions about sample collection still remain unanswered.
Question
How can a project team make sure that sampling strategy and microbiome methodology are well matched to the biological question before scaling up a larger study?
What we did
Biomcare supports clients early in project planning to help define the right microbiome approach from the start. This includes advising on study design, sequencing strategy, sample collection, and sample storage. When important uncertainties remain, we may recommend a pilot study to test sampling choices before a larger project begins. For example, this can help determine which sampling depth, distance, or collection strategy is most suitable for the biological question and study system.
Insight
A pilot can provide the data needed to validate both the sampling setup and the sequencing approach before committing to a large study. While it adds time and cost upfront, it can reduce the risk of generating inconclusive results later. By resolving key uncertainties early, the larger project can be designed with greater confidence and a stronger basis for producing useful biological insight.
"We relied heavily on the experience of Biomcare for help and advice on setting up the project and storing the samples."
