Step-by-Step: How to Collect Soil Samples for Microbiome Profiling
A practical guide to collecting reliable soil samples - whether you are new to soil biology testing or have done it before.
This guide will help you collect reliable soil samples for soil microbiome analysis - whether you are new to soil biology testing or have done it before.
Step 1: Contact Biomcare
Contact Biomcare to let us know you will be sending one or more samples. We will send you:
- Instructions regarding shipment address and current turnaround time
- A sample sheet for registering your sample labels and a few key details
For each batch of samples you send to us, you must email the completed sample sheet so we can keep track of your samples.
Step 2: Collect the sample(s)
Collect composite soil samples from your fields. You will need:
- A clean bucket
- A spoon or soil drill
- A small plastic bag or jar (a few ml in size)
- Labels for each sample
It is recommended to wear gloves. Do not handle the soil directly with your hands if you are not wearing gloves.
Collect 8-16 subsamples across the area you want to profile. Put the subsamples into the larger bucket and mix well. Remove any large clumps, stones, or organic material. Take a small uniform subsample into the labeled bag or jar and close it well. If using a jar, seal it with tape to ensure it stays closed during transportation.
The amount of composite soil we ask you to send is 3-5 spoons, or approximately a few ml.
Step 3: Send the sample(s)
When you first contact us, we will provide instructions on where to send your samples. Here are some best practices for shipping:
- Ensure bags or jars are tightly closed and will not leak during transit
- Use a shipping method that delivers samples to the lab within 2-3 days - overnight shipping is ideal
- Ship early in the week to avoid weekend delays
- At room temperature, total transit time should not exceed 5 days
- If outdoor temperatures exceed 20°C, include freeze elements in the shipment and use a polystyrene container
If you need to store samples before shipping:
- Store long-term at -20°C
- Store short-term (up to 7 days) at 0-6°C
Common questions
Do I need special equipment?
No special equipment is needed. A clean bucket, a spoon or soil drill, and a small labeled container are sufficient.
How deep should I sample?
We recommend sampling at a depth of 5-15 cm to represent both topsoil and lower layers. Depending on your aim, you can collect bulk soil only, rhizosphere soil only, or a combination. If collecting rhizosphere soil, pull up 8-16 plants, shake the soil from the roots, and mix it in the bucket. One composite sample should represent a uniform area with similar soil characteristics (pH, texture) and consistent management practices (fertilization, irrigation, etc.).
How long until I receive my results?
Turnaround time is usually 5-6 weeks from when samples arrive at our lab. See our FAQ for more details on delivery times.
Ready to get started?
Get in touch to discuss your project or request sample sheets.
