Microbiome profiling of animal models

Monitor your facility´s microbiome

The microbiota is a variable that can severely influence results from animal studies.

Animal models are essential for research and development in many fields of science. To obtain correct results in animal studies, detailed characterisation and control of the animal’s phenotype is necessary. Developments in recent years have shown that the composition of the gut microbiota is a variable that can influence animals’ phenotypes, and in turn severely alter study results.

Independent lineage of animals, both those bred in the same facility and those purchased from a vendor, display strong differences in their microbial communities. To control for this source of variation, it is necessary to monitor the animal’s microbiome.

Microbiome screening options

At Biomcare we offer multiple microbiome screening options that allow us to profile and monitor the microbiome of your animals at all timepoints and body sites. Together, we will identify the best setup for your facility, and design a sampling routine that fits into your daily routine and study flows.

Our services

  • Consultancy on sampling, project planning and results interpretation
  • DNA extraction and microbiome sequencing using NGS solutions
  • Data quality evaluation and processing
  • Biostatistical analysis
  • Data storage and handling

What cannot be controlled, must be monitored, and we are here to help you do just that.

Several techniques have been described for standardisation of the microbiota in animal models, and such approaches are key to ensure reproducibility of findings and avoids erroneous conclusions in studies where the microbiota can severely influence the results. For studies implementing such
techniques, it is necessary to ensure that the intended homogenisation is archived. Our microbiome profiling service allow you to evaluate and document the standardisation obtained from established protocols and to
monitor cross-generational and cage-specific microbiome profiles in the process of establishing your technique.

Factors influencing microbiome compositions

Microbiome in animal modelsThe factors influencing microbiome compositions are endless. Major sources of variation include vendor, strain, age, diet, single vs pair vs group-housed, type of bedding, recent transportation, health status, antimicrobial usage, and water source. It is impossible to remove all sources of variation. What cannot be controlled, must be monitored, and we are here to help you do just that.

At Biomcare, we are experts in microbiome data processing and biostatistical analysis. When you work with us to monitor your facility, we ensure that your data is maintained and organised for easy comparisons across time, animals, and studies. We work together to ensure a sample and information flow that suits your facility.

Partnering with Biomcare

Standardized workflow for microbiome profiling.

When you engage with Biomcare to monitor the microbiome of your animal facility, we establish a workflow that become part of your rutine. You never again have to understand new processes for storage, shipment, labelling, data retrieval, and so-forth. Obtaining microbiome profiles becomes easy and, importantly, all your data is stored and maintained by Biomcare, allowing for easy comparison of any samples ever profiled.

We use the latest reference databases and process all data with the best opensource software used within the international bioinformatic community

Biomcare handles all data processing from raw data to microbiome profiles, using our standardised workflows. It is out job to know the software, evaluate data quality and when updated solutions are available, to reanalyse all your data so that you always have a fully up-to-date data set, annotated with the latest reference databases and processed with best opensource software.

Opensource software pipelines

At Biomcare we believe in transparency. All our pipelines are build with opensource  software, and freely available reference databases. This is a cornerstone for research and allow you to easily include data and results from Biomcare in your publications.

We are actively engaged in research and when a new software has been thoroughly tested and is becoming the new gold standard in the international bioinformatic community, we start implementing it into
our pipelines, thereby keeping at the forefront of developments without jumping ahead with untested solutions.

All data in one place

We know how difficult it is to keep track of projects in an active research unit. Often, data generated for previous projects is not accessible for new projects. The data might be lost with changing staf, or incompatible due to differences in sampling, sequencing or processing protocols and providers.

When working with Biomcare,  standardisation ensure compatibility and our collected data storage ensure all data is ready for effective cross-sample  comparisons, so that the microbiome of new mice can easily be compared to animals that arrived from that vendor last month or last year.

Example of data analysis of animal models

Example of data analysis of animal models

Microbiome experts at hand

Working with Biomcare means that you always have a microbiome expert at hand, to ask your questions, whether it is about  sampling, project planning or interpretation of results.

In summary

When partnering with Biomcare we become your microbiome department. A one-contact point for all you need to monitor the
microbiome profiles of your study animals and implement microbiome insight in your studies.

  • Consultancy on sampling, project planning and results interpretation
  • DNA extraction and microbiome sequencing using NGS solutions
  • Data quality evaluation and processing
  • Biostatistical analysis
  • Data storage and handling

 

Solving your microbiome problems

We have more than +7 years of experience in analyzing microbiome data and structuring microbiome projects. Our team has worked on more than 50 microbiome projects spanning research, universities and commercial industries.

Biomcare offers both microbiome services for small discovery projects, as well as large custom-designed microbiome projects.

Contact us today and get a quote. We are standing by to service you.